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a fumigatus wild type wt strain  (ATCC)


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    ATCC a fumigatus wild type wt strain
    ( A to F ) BMDMs expressing sh_Ctrl or sh_ Rab5c were stimulated <t>with</t> <t>wild-type</t> (WT; ATCC46645) or Δ pksP A. <t>fumigatus</t> conidia. [(A) and (B)] Confocal images (A) and percentage (B) of LC3 + phagosomes. Insets: DIC of conidia. Scale bar, 10 μm. (C) Percentage of NBT + phagocytes. (D) Percentage of ATP6V1A + phagosomes after 1 hour. [(E) and (F)] Viability of WT (E) and Δ pksP (F) conidia. ( G and H ) Atg16l1 FL and Atg16l1 Δ WD40 BMDMs were stimulated with WT or Δ pksP conidia. Percentage of LC3 + phagosomes (G) and NBT + phagocytes (H). ( I to K ) Viability of WT conidia (I), Δ pksP conidia (J), and WT conidia ± DPI (5 μM) (K) in Atg16l1 FL and Atg16l1 Δ WD40 BMDMs. ( L ) Schematics of A. fumigatus lung infection. Created in BioRender. L. Cunha (2026), https://BioRender.com/l4k0vfu . h, hours. ( M ) Fungal loads in the bronchoalveolar lavage fluid (BAL) samples of infected mice. ( N ) Quantification of albumin in BAL. ( O ) Bright-field images of lung sections stained with Grocott-Gomori’s methenamine silver (top) or hematoxylin and eosin (bottom). Scale bar, 500 μm. Insets: A. fumigatus hyphae. Scale bar, 50 μm. ( P to S ) Quantification of interleukin-6 (IL-6) (P), CCL2 (Q), tumor necrosis factor–α (TNF-α) (R), and IL-1β (S) in BAL. Bars [(B) to (K), (M), (N), and (P) to (S)] represent means of biological replicates (sample or mice), each indicated as a white object. Error bars, ±SEM. Statistical comparison between groups are unpaired Student’s t test [(B) to (J), (M), (N), and (P) to (S)] or ANOVA and Tukey’s multiple comparisons (K) between indicated groups. Data are representative of two [(A) to (D), (G), (H), (J), (K), and (M) to (S)] or three [(E), (F), and (I)] independent experiments.
    A Fumigatus Wild Type Wt Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+fumigatus+wild+type+wt+strain/Aspergillus+fumigatus/pmc13155314-124-10-15
    Average 96 stars, based on 1516 article reviews
    a fumigatus wild type wt strain - by Bioz Stars, 2026-10
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    1) Product Images from "RAB5c orchestrates LC3-associated phagocytosis to promote microbicidal function of macrophages"

    Article Title: RAB5c orchestrates LC3-associated phagocytosis to promote microbicidal function of macrophages

    Journal: Science Advances

    doi: 10.1126/sciadv.adz0196

    ( A to F ) BMDMs expressing sh_Ctrl or sh_ Rab5c were stimulated with wild-type (WT; ATCC46645) or Δ pksP A. fumigatus conidia. [(A) and (B)] Confocal images (A) and percentage (B) of LC3 + phagosomes. Insets: DIC of conidia. Scale bar, 10 μm. (C) Percentage of NBT + phagocytes. (D) Percentage of ATP6V1A + phagosomes after 1 hour. [(E) and (F)] Viability of WT (E) and Δ pksP (F) conidia. ( G and H ) Atg16l1 FL and Atg16l1 Δ WD40 BMDMs were stimulated with WT or Δ pksP conidia. Percentage of LC3 + phagosomes (G) and NBT + phagocytes (H). ( I to K ) Viability of WT conidia (I), Δ pksP conidia (J), and WT conidia ± DPI (5 μM) (K) in Atg16l1 FL and Atg16l1 Δ WD40 BMDMs. ( L ) Schematics of A. fumigatus lung infection. Created in BioRender. L. Cunha (2026), https://BioRender.com/l4k0vfu . h, hours. ( M ) Fungal loads in the bronchoalveolar lavage fluid (BAL) samples of infected mice. ( N ) Quantification of albumin in BAL. ( O ) Bright-field images of lung sections stained with Grocott-Gomori’s methenamine silver (top) or hematoxylin and eosin (bottom). Scale bar, 500 μm. Insets: A. fumigatus hyphae. Scale bar, 50 μm. ( P to S ) Quantification of interleukin-6 (IL-6) (P), CCL2 (Q), tumor necrosis factor–α (TNF-α) (R), and IL-1β (S) in BAL. Bars [(B) to (K), (M), (N), and (P) to (S)] represent means of biological replicates (sample or mice), each indicated as a white object. Error bars, ±SEM. Statistical comparison between groups are unpaired Student’s t test [(B) to (J), (M), (N), and (P) to (S)] or ANOVA and Tukey’s multiple comparisons (K) between indicated groups. Data are representative of two [(A) to (D), (G), (H), (J), (K), and (M) to (S)] or three [(E), (F), and (I)] independent experiments.
    Figure Legend Snippet: ( A to F ) BMDMs expressing sh_Ctrl or sh_ Rab5c were stimulated with wild-type (WT; ATCC46645) or Δ pksP A. fumigatus conidia. [(A) and (B)] Confocal images (A) and percentage (B) of LC3 + phagosomes. Insets: DIC of conidia. Scale bar, 10 μm. (C) Percentage of NBT + phagocytes. (D) Percentage of ATP6V1A + phagosomes after 1 hour. [(E) and (F)] Viability of WT (E) and Δ pksP (F) conidia. ( G and H ) Atg16l1 FL and Atg16l1 Δ WD40 BMDMs were stimulated with WT or Δ pksP conidia. Percentage of LC3 + phagosomes (G) and NBT + phagocytes (H). ( I to K ) Viability of WT conidia (I), Δ pksP conidia (J), and WT conidia ± DPI (5 μM) (K) in Atg16l1 FL and Atg16l1 Δ WD40 BMDMs. ( L ) Schematics of A. fumigatus lung infection. Created in BioRender. L. Cunha (2026), https://BioRender.com/l4k0vfu . h, hours. ( M ) Fungal loads in the bronchoalveolar lavage fluid (BAL) samples of infected mice. ( N ) Quantification of albumin in BAL. ( O ) Bright-field images of lung sections stained with Grocott-Gomori’s methenamine silver (top) or hematoxylin and eosin (bottom). Scale bar, 500 μm. Insets: A. fumigatus hyphae. Scale bar, 50 μm. ( P to S ) Quantification of interleukin-6 (IL-6) (P), CCL2 (Q), tumor necrosis factor–α (TNF-α) (R), and IL-1β (S) in BAL. Bars [(B) to (K), (M), (N), and (P) to (S)] represent means of biological replicates (sample or mice), each indicated as a white object. Error bars, ±SEM. Statistical comparison between groups are unpaired Student’s t test [(B) to (J), (M), (N), and (P) to (S)] or ANOVA and Tukey’s multiple comparisons (K) between indicated groups. Data are representative of two [(A) to (D), (G), (H), (J), (K), and (M) to (S)] or three [(E), (F), and (I)] independent experiments.

    Techniques Used: Expressing, Infection, Staining, Comparison

    Related Articles

    Conjugation Assay:

    Article Title: RAB5c orchestrates LC3-associated phagocytosis to promote microbicidal function of macrophages
    Article Snippet: .. Silencing of Rab5c decreased LC3 conjugation to phagosomes containing the A. fumigatus wild-type (WT) strain [American Type Culture Collection (ATCC), 466645] ( ). ..



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    ( A to F ) BMDMs expressing sh_Ctrl or sh_ Rab5c were stimulated <t>with</t> <t>wild-type</t> (WT; ATCC46645) or Δ pksP A. <t>fumigatus</t> conidia. [(A) and (B)] Confocal images (A) and percentage (B) of LC3 + phagosomes. Insets: DIC of conidia. Scale bar, 10 μm. (C) Percentage of NBT + phagocytes. (D) Percentage of ATP6V1A + phagosomes after 1 hour. [(E) and (F)] Viability of WT (E) and Δ pksP (F) conidia. ( G and H ) Atg16l1 FL and Atg16l1 Δ WD40 BMDMs were stimulated with WT or Δ pksP conidia. Percentage of LC3 + phagosomes (G) and NBT + phagocytes (H). ( I to K ) Viability of WT conidia (I), Δ pksP conidia (J), and WT conidia ± DPI (5 μM) (K) in Atg16l1 FL and Atg16l1 Δ WD40 BMDMs. ( L ) Schematics of A. fumigatus lung infection. Created in BioRender. L. Cunha (2026), https://BioRender.com/l4k0vfu . h, hours. ( M ) Fungal loads in the bronchoalveolar lavage fluid (BAL) samples of infected mice. ( N ) Quantification of albumin in BAL. ( O ) Bright-field images of lung sections stained with Grocott-Gomori’s methenamine silver (top) or hematoxylin and eosin (bottom). Scale bar, 500 μm. Insets: A. fumigatus hyphae. Scale bar, 50 μm. ( P to S ) Quantification of interleukin-6 (IL-6) (P), CCL2 (Q), tumor necrosis factor–α (TNF-α) (R), and IL-1β (S) in BAL. Bars [(B) to (K), (M), (N), and (P) to (S)] represent means of biological replicates (sample or mice), each indicated as a white object. Error bars, ±SEM. Statistical comparison between groups are unpaired Student’s t test [(B) to (J), (M), (N), and (P) to (S)] or ANOVA and Tukey’s multiple comparisons (K) between indicated groups. Data are representative of two [(A) to (D), (G), (H), (J), (K), and (M) to (S)] or three [(E), (F), and (I)] independent experiments.
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    strains a fumigatus atcc 204305 wild type wt  (ATCC)
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    ( A to F ) BMDMs expressing sh_Ctrl or sh_ Rab5c were stimulated <t>with</t> <t>wild-type</t> (WT; ATCC46645) or Δ pksP A. <t>fumigatus</t> conidia. [(A) and (B)] Confocal images (A) and percentage (B) of LC3 + phagosomes. Insets: DIC of conidia. Scale bar, 10 μm. (C) Percentage of NBT + phagocytes. (D) Percentage of ATP6V1A + phagosomes after 1 hour. [(E) and (F)] Viability of WT (E) and Δ pksP (F) conidia. ( G and H ) Atg16l1 FL and Atg16l1 Δ WD40 BMDMs were stimulated with WT or Δ pksP conidia. Percentage of LC3 + phagosomes (G) and NBT + phagocytes (H). ( I to K ) Viability of WT conidia (I), Δ pksP conidia (J), and WT conidia ± DPI (5 μM) (K) in Atg16l1 FL and Atg16l1 Δ WD40 BMDMs. ( L ) Schematics of A. fumigatus lung infection. Created in BioRender. L. Cunha (2026), https://BioRender.com/l4k0vfu . h, hours. ( M ) Fungal loads in the bronchoalveolar lavage fluid (BAL) samples of infected mice. ( N ) Quantification of albumin in BAL. ( O ) Bright-field images of lung sections stained with Grocott-Gomori’s methenamine silver (top) or hematoxylin and eosin (bottom). Scale bar, 500 μm. Insets: A. fumigatus hyphae. Scale bar, 50 μm. ( P to S ) Quantification of interleukin-6 (IL-6) (P), CCL2 (Q), tumor necrosis factor–α (TNF-α) (R), and IL-1β (S) in BAL. Bars [(B) to (K), (M), (N), and (P) to (S)] represent means of biological replicates (sample or mice), each indicated as a white object. Error bars, ±SEM. Statistical comparison between groups are unpaired Student’s t test [(B) to (J), (M), (N), and (P) to (S)] or ANOVA and Tukey’s multiple comparisons (K) between indicated groups. Data are representative of two [(A) to (D), (G), (H), (J), (K), and (M) to (S)] or three [(E), (F), and (I)] independent experiments.
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    Image Search Results


    ( A to F ) BMDMs expressing sh_Ctrl or sh_ Rab5c were stimulated with wild-type (WT; ATCC46645) or Δ pksP A. fumigatus conidia. [(A) and (B)] Confocal images (A) and percentage (B) of LC3 + phagosomes. Insets: DIC of conidia. Scale bar, 10 μm. (C) Percentage of NBT + phagocytes. (D) Percentage of ATP6V1A + phagosomes after 1 hour. [(E) and (F)] Viability of WT (E) and Δ pksP (F) conidia. ( G and H ) Atg16l1 FL and Atg16l1 Δ WD40 BMDMs were stimulated with WT or Δ pksP conidia. Percentage of LC3 + phagosomes (G) and NBT + phagocytes (H). ( I to K ) Viability of WT conidia (I), Δ pksP conidia (J), and WT conidia ± DPI (5 μM) (K) in Atg16l1 FL and Atg16l1 Δ WD40 BMDMs. ( L ) Schematics of A. fumigatus lung infection. Created in BioRender. L. Cunha (2026), https://BioRender.com/l4k0vfu . h, hours. ( M ) Fungal loads in the bronchoalveolar lavage fluid (BAL) samples of infected mice. ( N ) Quantification of albumin in BAL. ( O ) Bright-field images of lung sections stained with Grocott-Gomori’s methenamine silver (top) or hematoxylin and eosin (bottom). Scale bar, 500 μm. Insets: A. fumigatus hyphae. Scale bar, 50 μm. ( P to S ) Quantification of interleukin-6 (IL-6) (P), CCL2 (Q), tumor necrosis factor–α (TNF-α) (R), and IL-1β (S) in BAL. Bars [(B) to (K), (M), (N), and (P) to (S)] represent means of biological replicates (sample or mice), each indicated as a white object. Error bars, ±SEM. Statistical comparison between groups are unpaired Student’s t test [(B) to (J), (M), (N), and (P) to (S)] or ANOVA and Tukey’s multiple comparisons (K) between indicated groups. Data are representative of two [(A) to (D), (G), (H), (J), (K), and (M) to (S)] or three [(E), (F), and (I)] independent experiments.

    Journal: Science Advances

    Article Title: RAB5c orchestrates LC3-associated phagocytosis to promote microbicidal function of macrophages

    doi: 10.1126/sciadv.adz0196

    Figure Lengend Snippet: ( A to F ) BMDMs expressing sh_Ctrl or sh_ Rab5c were stimulated with wild-type (WT; ATCC46645) or Δ pksP A. fumigatus conidia. [(A) and (B)] Confocal images (A) and percentage (B) of LC3 + phagosomes. Insets: DIC of conidia. Scale bar, 10 μm. (C) Percentage of NBT + phagocytes. (D) Percentage of ATP6V1A + phagosomes after 1 hour. [(E) and (F)] Viability of WT (E) and Δ pksP (F) conidia. ( G and H ) Atg16l1 FL and Atg16l1 Δ WD40 BMDMs were stimulated with WT or Δ pksP conidia. Percentage of LC3 + phagosomes (G) and NBT + phagocytes (H). ( I to K ) Viability of WT conidia (I), Δ pksP conidia (J), and WT conidia ± DPI (5 μM) (K) in Atg16l1 FL and Atg16l1 Δ WD40 BMDMs. ( L ) Schematics of A. fumigatus lung infection. Created in BioRender. L. Cunha (2026), https://BioRender.com/l4k0vfu . h, hours. ( M ) Fungal loads in the bronchoalveolar lavage fluid (BAL) samples of infected mice. ( N ) Quantification of albumin in BAL. ( O ) Bright-field images of lung sections stained with Grocott-Gomori’s methenamine silver (top) or hematoxylin and eosin (bottom). Scale bar, 500 μm. Insets: A. fumigatus hyphae. Scale bar, 50 μm. ( P to S ) Quantification of interleukin-6 (IL-6) (P), CCL2 (Q), tumor necrosis factor–α (TNF-α) (R), and IL-1β (S) in BAL. Bars [(B) to (K), (M), (N), and (P) to (S)] represent means of biological replicates (sample or mice), each indicated as a white object. Error bars, ±SEM. Statistical comparison between groups are unpaired Student’s t test [(B) to (J), (M), (N), and (P) to (S)] or ANOVA and Tukey’s multiple comparisons (K) between indicated groups. Data are representative of two [(A) to (D), (G), (H), (J), (K), and (M) to (S)] or three [(E), (F), and (I)] independent experiments.

    Article Snippet: Silencing of Rab5c decreased LC3 conjugation to phagosomes containing the A. fumigatus wild-type (WT) strain [American Type Culture Collection (ATCC), 466645] ( ).

    Techniques: Expressing, Infection, Staining, Comparison

    TABLE 2.

    Journal:

    Article Title: Rapid Detection of Triazole Antifungal Resistance in Aspergillus fumigatus ▿

    doi: 10.1128/JCM.02330-07

    Figure Lengend Snippet: TABLE 2.

    Article Snippet: Moreover, 10 A. fumigatus with known cyp 51A mutations (G54V, G54E, G54R, G54W, TR-L98H, G138C, M220V, M220K, M220T, and M220I) and 3 A. fumigatus wild-type (WT) strains (ATCC 13073, Af293, and AfR21) were used to validate the probes in this work.

    Techniques: Comparison, DNA Sequencing, In Vitro